| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Dopamine ELISA kit - Urine/Plasma - Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Dopamine levels in plasma and urine samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | 10 µl urine, 300 µl plasma |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 4.5 - 2 000 ng/ml |
| Sensitivity | 4.5 ng/ml urine, 25 pg/ml plasma |
| Specificity | No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | 4 hours |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
| Sample preparation | Sample preparation, extractiion and acylation (65min) |
| ELISA | Dopamine antiserum incubation (2H), revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Gaber EW & El-Attar HA. The relationship between dopamine and renalase in type 2 diabetic patients with and without diabetic nephropathy. British Journal of Diabetes & Vascular Disease May/June 2013 vol. 13 no. 3 130-137
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