商品介绍
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Histamine release kit - Whole blood samples |
| Description | Supplementary kit to in vitro diagnostics enzyme immunoassay (ELISA) for the quantitative determination of histamine release in whole blood samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | 500 µl whole blood |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 0.5 - 50 ng/ml |
| Sensitivity | 0.2 ng/ml |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
| Assay time | 1h30 sample preparation + ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | 24-hours heparinized whole blood stored at 20-25°C. |
| Sample preparation | Sample acylation (1h30) |
| ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
- Chirumbolo S. et al. Bimodal action of the flavonoid quercetin on basophil function: an investigation of the putative biochemical targets. Clin Mol Allergy. 2010 Sep 17;8:13.
- Martin SK et al. Influence of Dietary Histidine on Basophil Release, Circulating Concentration, and Urinary Excretion of Histamine in Domestic Felines. Intern J Appl Res Vet Med. 2012. 10: 289-299
- McCord JL et al. H2-receptor-mediated vasodilation contributes to postexercise hypotension. J Appl Physiol (1985). 2006 Jan;100(1):67-75
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