immusmol/Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack<br />(3-KYNs ELISA pack)/3 x 96-well plate/ISE-220102,immusmol,,immusmol,+,,Array.Array.Array蚂蚁淘厂家直采.

产品编号：ISE-220102

市场价：¥30000.00

场地：美国(厂家直采)

产品分类：仪器类>影像显示>显微镜>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1500.00

品牌： immusmol

公司：immusmol

公司分类：

商品介绍

Product overview

Product name	Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack
Description	Three enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine, Kynurenic acid and Quinolinic acid in serum samples.
Labels	CE
Format	3 x 96-well plate
Samples	Serum
Minimum sample volume	20-50 µL
Cross specie-reactivity	React with all species
Standard range	KYN: 100 - 10000 ng/mlKYNA: 1.40 - 74 ng/ml QA: 200 - 2000 µg/ml
Sensitivity	LoD KYN: 47.5 ng/mlLoD KYNA: 0.53 ng/ml LoD QA: 6 ng/ml
Specificity	No significant cross-reactivity was observed analogs for each kit. See product pages for L-Kynurenine ELISA, Kynurenic acid ELISA and Quinolinic acid ELISA.
Storage	Store at 2-8°C for to 6 months.
Datasheets	Instructions for use - KYN kit, Instructions for use - KYNA kit, Instructions for use - QA kit

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Key steps

Sample collection & storage	Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation	KYN: Sample acylation (90 min) KYNA: Sample derivatization (90 min) QA: Sample acylation (120 min)
ELISA	Antisera overnight incubation, revelation and read steps (1h).
Detailed protocol	Instructions for use - KYN kit, Instructions for use - KYNA kit, Instructions for use - QA kit

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References

KYN ELISA citations :

Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.

Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.

Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3

Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4

Selected articles on Kynurenic acid:

Pedraz-Petrozzi et al. Effects of inflammation on the kynurenine pathway in schizophrenia - a systematic review. J Neuroinflammation. 2020
Achtyes et al. Inflammation and kynurenine pathway dysregulation in post-partum women with severe and suicidal depression. Brain Behav Immun. 2020
Walczak et al. Kynurenic acid and cancer: facts and controversies. Cell Mol Life Sci. 2019
Lim et al. Kynurenine pathway metabolomics predicts and provides mechanistic insight into multiple sclerosis progression. Sci Rep. 2017
Plitman et al. Kynurenic Acid in Schizophrenia: A Systematic Review and Meta-analysis. Schizophr Bull. 2017
Wirthgen et al. Kynurenic Acid: The Janus-Faced Role of an Immunomodulatory Tryptophan Metabolite and Its Link to Pathological Conditions. Front Immunol. 2017

Selected articles on Quinolinic acid:

Fertan et al. Effects of the Novel IDO Inhibitor DWG-1036 on the Behavior of Male and Female 3xTg-AD Mice. Front Pharmacol. 2019 Sep;24

Pierozan et al. Synergistic Toxicity of the Neurometabolites Quinolinic Acid and Homocysteine in Cortical Neurons and Astrocytes: Implications in Alzheimer"s Disease. Neurotox Res. 2018 Jul;34

Hernandez-Martinez et al. Quinolinic acid induces neuritogenesis in SH-SY5Y neuroblastoma cells independently of NMDA receptor activation. Eur J Neurosci. 2017 Mar;45

Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13