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| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name | Quinolinic Acid ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Quinolinic Acid in serum and plasma samples. For research use only. |
| Format | 96-well plate |
| Samples | Serum, Plasma |
| Minimal sample volume | 25 µL |
| Species reactivity | React with all species |
| Standard range | 25 - 2000 ng/mL |
| Sensitivity | < 6 ng/mL |
| Specificity | No significant cross-reactivity was observed with Quinolinic Acid analogs such as Kynurenic acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid |
| Assay time | Sample acylation 120 min and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Plasma (Heparin) Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation | Sample acylation (120 min) |
| ELISA | Quinolinic acid antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
Selected articles on Quinolinic acid:
- Fertan et al. Effects of the Novel IDO Inhibitor DWG-1036 on the Behavior of Male and Female 3xTg-AD Mice. Front Pharmacol. 2019 Sep;24
- Pierozan et al. Synergistic Toxicity of the Neurometabolites Quinolinic Acid and Homocysteine in Cortical Neurons and Astrocytes: Implications in Alzheimer"s Disease. Neurotox Res. 2018 Jul;34
- Hernandez-Martinez et al. Quinolinic acid induces neuritogenesis in SH-SY5Y neuroblastoma cells independently of NMDA receptor activation. Eur J Neurosci. 2017 Mar;45
- Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13
- Guillemin GJ. Quinolinic acid, the inescapable neurotoxin. FEBS J. 2012 Apr;279
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