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Product overview
| Product name | Metanephrine ELISA kit - Urine- Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of metanephrine (metadrenaline) in urine samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | Urine |
| Sample volume | 25 µL |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 20 - 2 000 ng/ml |
| Sensitivity | 13 ng/ml |
| Specificity | No significant cross-reactivity was observed with Metanephrine analogs such as Normetanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine. |
| Assay time | Sample hydrolysis and acetylation 45 min and ELISA 1h |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage | Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months) |
| Sample preparation | Sample hydrolysis (30 min) and acetylation (15min) |
| ELISA | Metanephrine antiserum incubation (30 min), revelation and read steps (30h). |
| Detailed protocol | Download instructions for use |
References
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