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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
immusmol/3 Catecholamines ELISA kit - Urine & Plasma - Fast/BA-E-6600
产品编号:BA-E-6600
市  场 价:¥23400.00
场      地:美国(厂家直采)
产品分类: 蛋白类>>激酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1170.00
品      牌: immusmol
公      司:immusmol
公司分类:
immusmol/3 Catecholamines ELISA kit - Urine & Plasma - Fast/BA-E-6600
商品介绍
Product informationKey stepsReferencesTechnical supportReviews

Product overview

Product name

3 catecholamines ELISA kit - Urine/Plasma - Fast-Track

DescriptionIn vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Adrenaline, Noradrenaline and Dopamine levels in plasma and urine samples.
LabelsIVD, CE
Format3x96-well plate
SamplesUrine, Plasma
Sample volume10 µl urine, 300 µl plasma
Cross specie-reactivityReact with all species
Standard rangeEpinephrine: 0 / 1 - 200 ng/ml Norepinephrine: 0 / 5 - 1 000 ng/ml Dopamine: 0 / 4.5 - 2 000 ng/ml
Sensitivity

Epinephrine: 0.3 ng/ml urine, 10 pg/ml plasma Norepinephrine: 1.5 ng/ml urine, 40 pg/ml plasma Dopamine: 4.5 ng/ml urine, 25 pg/ml plasma

SpecificityNo significant cross-reactivity was observed with Dopamine, Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.
Assay time4 hours
StorageStore at 2-8°C for to 6 months.
DatasheetsInstructions for useMaterial safety datasheet
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Key steps

Sample collection & storage

Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C.

Sample preparation

Sample preparation, extraction and acylation (65min)

ELISADopamine, Epinephrine, Norepinephrine antisera incubation (2H), revelation and read steps (1h).
Detailed protocolDownload instructions for use
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References

  • Product citation: Chennupati et al. Myogenic vasoconstriction requires G12/G13 and LARG to maintain local and systemic vascular resistance. Elife. 2019 Sep 24

 

  • Product citation: Elmarakby et al. Increased arterial pressure in mice with overexpression of the ADHD candidate gene calcyon in forebrain. PLoS One. 2019 Feb 12

 

  • Product citation: Boisvert et al. Hyperfiltration in Ubiquitin C-Terminal Hydrolase L1-Deleted Mice. Clin Sci (Lond). 2018 Jul 18;132(13)

 

  • Kang SW et al. Renal kallikrein excretion and epigenetics in human acute kidney injury: expression, mechanisms and consequences. BMC Nephrol. 2011 Jun 16;12:27.
  • Dundon CM &  Rellini AH. Emotional states of love moderate the association between catecholamines and female sexual responses in the laboratory. J Sex Med. 2012 Oct;9(10):2617-30.
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