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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
immusmol/Epinephrine - Norepinephrine ELISA kit - Research/BA-E-5400
产品编号:BA-E-5400
市  场 价:¥15920.00
场      地:美国(厂家直采)
产品分类: 抗体类>二抗>抗生物素>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$796.00
品      牌: immusmol
公      司:immusmol
公司分类:
immusmol/Epinephrine - Norepinephrine ELISA kit - Research/BA-E-5400
商品介绍
Product informationKey stepsReferencesTechnical supportReviews

Product overview

Product name

Epinephrine-Norepinephrine ELISA kit - Research

DescriptionEnzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only.
Format2 x 96-well plate
SamplesAny sample
Sample volume1 - 750 µl
Cross specie-reactivityReact with all species
Standard rangeEpinephrine: 0 / 0.5 - 80 ng/ml;Norepinephrine: 0 / 0.2 - 32 ng/ml
Sensitivity

Epinephrine: 6.6 pg/ml; Norepinephrine: 2.6 pg/ml

SpecificityNo significant cross-reactivity was observed with Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.
Assay timeSample preparation 4 hours and ELISA overnight
StorageStore at 2-8°C for to 6 months.
DatasheetsInstructions for useMaterial safety datasheet
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Key steps

Sample preparation

Sample extraction and acylation (1h45), enzymatic conversion (2h)

ELISAEpinephrine/Norepinephrine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocolDownload instructions for use
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References

  • El Khoury N et al. Upregulation of the hyperpolarization-activated current increases pacemaker activity of the sinoatrial node and heart rate during pregnancy in mice. Circulation. 2013 May 21;127(20):2009-20.
  • Jun JC et al. Acute hypoxia induces hypertriglyceridemia by decreasing plasma triglyceride clearance in mice. Am J Physiol Endocrinol Metab. 2012 Aug 1;303(3):E377-88.
  • Al-Wadei M. and Schuller HM. Pancreatic cancer cells and normal pancreatic duct epithelial cells express an autocrine catecholamine loop that is activated by nicotinic acetylcholine receptors α3, α5 and α7. Mol Cancer Res. Feb 2012; 10(2): 239–249.
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