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Product overview
Product name | Metanephrine-Normetanephrine ELISA kit - Urine- Fast-Track |
Description | In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of metanephrines (metadrenaline and normetadrenaline) in urine samples. |
Labels | IVD, CE |
Format | 96-well plate |
Samples | Urine |
Sample volume | 25 µL |
Cross specie-reactivity | React with all species |
Standard range | 0 / 20 - 2 000 ng/ml - Metanephrine0 / 30 - 3 000 ng/ml - Normetanephrine |
Sensitivity | 13 ng/ml - Metanephrine23 ng/ml - Normetanephrine |
Specificity | No significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine. |
Assay time | Sample hydrolysis and acetylation 45 min and ELISA (1) |
Storage | Store at 2-8°C for to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample collection & storage | Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months) |
Sample preparation | Sample hydrolysis (30 min) and acetylation (15min) |
ELISA | Metanephrine antiserum incubation (30 min), revelation and read steps (30h). |
Detailed protocol | Download instructions for use |
References
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