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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
immusmol/L-Tryptophan ELISA kit/BA-E-2700
产品编号:BA-E-2700
市  场 价:¥7800.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$390.00
品      牌: immusmol
公      司:immusmol
公司分类:
immusmol/L-Tryptophan ELISA kit/BA-E-2700
商品介绍
Product informationKey stepsReferencesTechnical supportReviews

Product overview

Product name

Tryptophan ELISA kit

DescriptionIn vitro diagnostic enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, ...) and cell culture supernatants.
LabelsIVD, CE
Format96-well plate
Samplesurine, plasma, serum, cell culture supernatant
Sample volume20 µL
Cross species-reactivityReact with all species
Standard range2.5 - 250 µg/ml
Sensitivity

< 1.2µg/ml

Specificity

No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine.

Assay timeSample preparation 2.5 hours and ELISA overnight
StorageStore at 2-8°C for to 6 months.
DatasheetsInstructions for useMaterial safety datasheet
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Key steps

Sample collection & storage

Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples.

Sample preparation

Sample precipitation (15 min) and derivatization (2h15)

ELISATryptophan antiserum overnight incubation, revelation and read steps (1h).
Detailed protocolDownload instructions for use
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References

PrProduct citations :

 

  • Watanabe et al.Addding indoximod to hypofractionated radiotherapy with anti-PD-1 checkpoint blockade enhances early NK and CD8+ T cell-dependent tumor activity. Clin Cancer Res. 2019 Nov 6

 

  • Walker et al. Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice. Mol Psychiatry. 2018 Jul 9.

 

  • Ziklo et al. Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections. Front Cell Infect Microbiol. 2018 Jan 18.

 

  • Gautam et al. In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2018 Jan 2

 

  • Martiin-Hernandez et al. Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats. Mol Neurobiol. 2018 May 3

 

  • Toulmonde et al. Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas. JAMA Oncol. 2018 Jan 1;4
  • Ziklo et al. In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp. BMC Microbiol. 2016 Dec 3.
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