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Product overview
Product name | Tryptophan ELISA kit |
Description | In vitro diagnostic enzyme immunoassay (ELISA) for the quantitative determination of L-Tryptophan in biological fluids (urine, plasma, serum samples, ...) and cell culture supernatants. |
Labels | IVD, CE |
Format | 96-well plate |
Samples | urine, plasma, serum, cell culture supernatant |
Sample volume | 20 µL |
Cross species-reactivity | React with all species |
Standard range | 2.5 - 250 µg/ml |
Sensitivity | < 1.2µg/ml |
Specificity | No significant cross-reactivity was observed with L-Tryptophan analogs such as 5-Hydroxy-L-tryptophan, Tryptamine, 5-Methoxytryptophan, 5-Hydroxytryptamine, N-acetyl-5-hydroxytryptamine. |
Assay time | Sample preparation 2.5 hours and ELISA overnight |
Storage | Store at 2-8°C for to 6 months. |
Datasheets | Instructions for use , Material safety datasheet |
Key steps
Sample collection & storage | Urine: spontaneous or 24-hour urine collected in bottle with 10-15 mL of 6 M HCl, stored at -20°C for long periods (up to 6 months)Plasma/Serum: EDTA-, Heparin- or Citrate- samples stored at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). Do not use haemolytic or lipemic samples. |
Sample preparation | Sample precipitation (15 min) and derivatization (2h15) |
ELISA | Tryptophan antiserum overnight incubation, revelation and read steps (1h). |
Detailed protocol | Download instructions for use |
References
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